Clean technique for cryoprotectant-free vitrification of human spermatozoa
نویسندگان
چکیده
منابع مشابه
Large volume cryoprotectant-free vitrification: an alternative to conventional cryopreservation for human spermatozoa.
Vitrification is a simple and cost-effective method for the storage of human spermatozoa without the use of conventional cryoprotectants, by plunging the sperm suspension directly into liquid nitrogen. As a result, solidification of living cells without the formation of ice crystals is achieved during cooling. This study aimed to compare cryoprotectant-free vitrification to conventional cryopre...
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Recent studies have shown that L-proline is a natural osmoprotectant and an antioxidant to protect cells from injuries such as that caused by freezing and thawing in many species including plant, ram sperm and human endothelial cells. Nevertheless, this nontoxic cryoprotectant has not yet been applied to mammalian oocyte vitrification. In this study we evaluated the efficiency and safety of the...
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Cryopreservation of human spermatozoa free from cryoprotectant can avoid toxicity caused by highly concentrated cryoprotectant and a series of specific carriers have been previously explored, except for PDMS chip. Our study is aimed at exploring a novel device for ultra-rapid cryopreservation of small numbers of spermatozoa without cryoprotectant based on polydimethylsiloxane (PDMS) chips. Sper...
متن کاملvitrification increased vacuolization of human spermatozoa: application of msome technology
background: sperm vitrification is a technique of ice and cryoprotectant free cryopreservation by direct plunging of sperm suspension into liquid nitrogen (ln2). the aim of this study was to investigate the influence of cryoprotectant free-vitrification on human sperm fine structure by msome technology and the fertility potential by zona binding assay (zba). methods: 20 normo-ejaculates were pr...
متن کاملP-150: Effect of Vitrification on High Magnification Morphology and Fertility Potential of Human Spermatozoa Cell
Background: Many sperm characteristics including, motility and morphology are important for normal spermatozoa- oocyte intraction. These features may be altered during vitrification. Motile sperm organelle morphology examination (MSOME) is an unstained real time high magnification technique that analysis viable sperm morphology instantly. This study aimed to investigate the influence of vitrifi...
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ژورنال
عنوان ژورنال: Reproductive BioMedicine Online
سال: 2005
ISSN: 1472-6483
DOI: 10.1016/s1472-6483(10)61795-6